ProjectsLab software

BCA Analyzer: plate reader to protein concentration

v1.0LiveStreamlitPython

A BCA assay is the most routine measurement in a protein lab, and it usually ends in a hand-built spreadsheet. BCA Analyzer replaces the spreadsheet: upload the plate reader's export, click wells to mark standards and samples, and read off concentrations and dilution volumes.

BCA Analyzer's plate editor: a 96-well plate with seven standards and a blank in duplicate, and 24 samples, each well showing its absorbance. — The plate editor, with synthetic data.

01What it does

Reads the instrument's file. Exports from Tecan, BioTek Gen5, and SoftMax Pro, with the plate layout detected automatically.
One plate map. Assign standards, samples, and blanks by clicking or dragging across wells.
Curve and QC. Fits the standard curve, flags replicates that disagree and samples outside the standard range, and shows the math.
Dilution prep. Solves for the volumes to bring every sample to a target concentration, as a printable table.
Reports. Excel and PDF exports for the notebook.

02Built with

Python, Streamlit, a custom JavaScript plate-grid component, pytest, and Playwright end-to-end tests.

03Status

Version 1.0, in use in the lab and live on Streamlit Community Cloud. Try it with any Tecan, Gen5, or SoftMax export. The code is private.